카드뮴(Cd)의 세포독성에 대한 tannic acid의 해독 효과
ANTITOXIC EFFECTS OF TANNIC ACID AGAINST CADMIUM CYTOTOXICITY
소속 상세정보
1한두석/1Du Seok Han
2백승화/2Seung Hwa Baek
KMID : 0355819960200010065
Abstract
-Abstract-
This study was carried out to develop compounds repairing rat fibroblast and
neuroglial cells damaged by cadmium in vitro. The experimental groups devided into six
groups in tannic acid ; control group was cultured in the media only,
NR50 and MTT50 groups were cultured in the media
containing NR50 of cadmium plus various concentrations of tannic acid.
The 5.0×104 cell/㎖ of rat fibroblasts and neuroglial cells each well of
24 multidish were cultured. After 24 hours, the cells were treated with solution of all
groups. After the fibroblast and neuroglial cells of all groups were cultured in same
condition for 48 hours. Neutral red (NR) and tetrazolium MTT (MTT) of the
colorimetric assay were performed to evaluate the cytotoxicity of cell orgenelles. Also,
the lactate dehydrogenase (LDH) activity was measured for membrane toxicity. The
light microscopic study was carried out to observe morphological changes of cultured rat
fibroblast and neuroglial cells.
The results were as follows :
1. According to the NR absorbance, the effects of tannic acid 50 μM concentration were
active in fibroblast and neuroglial cells. but 100 μM tannic acids were decreased effects
again.
2. By means of MTT absorbance, the antitoxic effects of 50 μM tannic acid
concentration were active in fibroblast and neuroglial cells. However, 100 μM tannic
acids were decreased repaired effects again.
3. In LDH activity, tannic acid 50 μM concentration had good antitoxic effects in
fibroblast and neuroglial cells but 100 μM tannic acids were decreased antitoxic effects
again.
4. The cell numbers and cell shapes were repaired in the tannic acid 25 μM and 50 μ
M concentration, but 100 μM of tannic acids showed degeneration again.
These results indicated that tannic acid decreased the cytotoxicity of cadmium in
cultured fibroblast and neuroglial cells.
키워드
원문 및 링크아웃 정보
등재저널 정보